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KMID : 0613820210310020192
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Journal of Life Science
2021 Volume.31 No. 2 p.192 ~ p.198
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Evaluation of Lipid Accumulation’s Inhibitory Activity on 3T3-L1 Cells with Red Yeast Barley Extracts
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Kwon Gi-Seok
Kim Byung-Hyuk
Lee Jun-Hyeong
Hwang Hak-Soo
Lee Jung-Bok
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Abstract
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Red yeast rice has been extensively used as food and traditional medicine for thousands of years in East Asian countries. It is produced by the fermentation of a particular yeast (in general, Monascus purpureus) as rice and various cereals (barley, soybean, etc.). Monascus sp. produces many secondary metabolites during its growth, including pigments, monacolins, and γ-aminobutyric acid. Some metabolites?specifically, monacolin K, γ-aminobutyric acid, dimerumic acid, and monascus pigments?have been reported to lower cholesterol and blood pressure while showing anti-obesity effects. In this study, we investigated the anti-obesity effect of ethanol extract from red yeast barley (RYB) fermented with Monascus sp. BHN-MK 2 on 3T3-L1 cells. The anti-obesity effects of RYB extract were examined: its lipid accumulation inhibitory effect was tested by Oil Red O staining, and obesity-related mRNA expression levels were tested by real-time RT-PCR in MDI stimulated 3T3-L1 cells. The intracellular lipid content of MDI-stimulated 3T3-L1 cells decreased significantly to 5.04%, 12.24%, and 23.52% in response to 200, 400, and 800 μg/ml RYB, respectively. Moreovers, we evaluated that RYB extract significantly downregulated the expression of C/EBPα, SREBP-1, and PPAR-γ gene in a dose-dependent manner. As a result, red yeast barley ethanol extracts exerted the strongest anti-obesity effects. Also, the results indicate that red yeast barley could be used as a functional anti-obesity food material.
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KEYWORD
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3T3-L1, bioconversion, fermentation, Monascus sp., red-yeast barley
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